Part:BBa_K4767006

Pars-RBS-arsR-RBS-cymA-TT

Uses a factor P_ars(BBa_K4767001) ,strong RBS(BBa_J34801), DNA binding transcriptional repressor arsR(BBa_J15101), c-type cytochromes cymA(BBa_K2706008) and double terminator TT(BBa_B0015). This part is an easy BioBrick.

Usage and Biology

This part is a arsenic reporter with the reporter gene cymA. CymA is a c-type cytochromes from Shewanella oneidensis. It plays an important role in its extracellular electron transfer. To verify this part can respond to arsenic, we transfer this part into a S. oneidensis strain with the cymA gene mutant. Then we cultured the strain with a arsenic concentration gradient. When arsenic is absent, the transcription regulator ArsR binds to the ArsR-binding site (ABS) within the ars promoter and blocks transcription. Once arsenic is present, it binds to ArsR and activate the transcription of the cymA genes. As the result showed, strains with higher arsenic has a faster iron reduction rate, indicating the more expression of cymA.

Fig. Iron reduction curves with different arsenic concentrations.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 270
  Illegal BglII site found at 824
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References

Xiaoqiang Jia, Bu Rongrong, Zhao Tingting, et al. Sensitive and Specific Whole-Cell Biosensor for Arsenic Detection[J]. Applied and environmental microbiology, 2019, 85(11): 1.